Magnesium in PDB 1sa1: Tubulin-Podophyllotoxin: Stathmin-Like Domain Complex

The structure of Tubulin-Podophyllotoxin: Stathmin-Like Domain Complex, PDB code: 1sa1 was solved by R.B.Ravelli, B.Gigant, P.A.Curmi, I.Jourdain, S.Lachkar, A.Sobel, M.Knossow, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	20.00 / 4.20
Space group	P 65
Cell size a, b, c (Å), α, β, γ (°)	328.061, 328.061, 54.301, 90.00, 90.00, 120.00
R / Rfree (%)	20.4 / 25.9

The binding sites of Magnesium atom in the Tubulin-Podophyllotoxin: Stathmin-Like Domain Complex (pdb code 1sa1). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Tubulin-Podophyllotoxin: Stathmin-Like Domain Complex, PDB code: 1sa1:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the Tubulin-Podophyllotoxin: Stathmin-Like Domain Complex


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Tubulin-Podophyllotoxin: Stathmin-Like Domain Complex within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg501 b:0.7 occ:1.00 O3G A:GTP600 1.7 1.0 1.0 O2G A:GTP600 2.0 0.0 1.0 PG A:GTP600 2.2 0.3 1.0 O2B A:GTP600 2.4 0.2 1.0 O3B A:GTP600 2.9 0.1 1.0 PB A:GTP600 3.0 0.4 1.0 CA A:GLY144 3.3 0.6 1.0 N A:GLY144 3.3 0.6 1.0 N A:THR145 3.4 0.6 1.0 O1G A:GTP600 3.5 0.6 1.0 O3A A:GTP600 3.6 0.6 1.0 CG2 A:THR145 3.7 0.8 1.0 C A:GLY144 3.9 0.6 1.0 N A:ASN101 4.0 0.3 1.0 C A:GLY143 4.1 0.6 1.0 CB A:ALA100 4.2 0.1 1.0 C A:ALA99 4.2 0.4 1.0 ND2 A:ASN101 4.3 0.4 1.0 OG1 A:THR145 4.3 0.6 1.0 O A:ALA99 4.4 0.3 1.0 N A:ALA100 4.4 0.3 1.0 O1B A:GTP600 4.4 0.5 1.0 CB A:THR145 4.4 0.7 1.0 CB A:ALA99 4.5 0.2 1.0 CA A:THR145 4.5 0.6 1.0 CA A:ASN101 4.5 0.3 1.0 CG A:ASN101 4.6 0.2 1.0 CA A:ALA100 4.6 0.2 1.0 C A:ALA100 4.6 0.2 1.0 O A:GLY143 4.6 0.5 1.0 CA A:ALA99 4.7 0.4 1.0 OD1 A:ASN101 5.0 0.9 1.0 CA A:GLY143 5.0 0.5 1.0

Magnesium binding site 2 out of 2 in the Tubulin-Podophyllotoxin: Stathmin-Like Domain Complex


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Tubulin-Podophyllotoxin: Stathmin-Like Domain Complex within 5.0Å range: probe atom residue distance (Å) B Occ C:Mg502 b:0.1 occ:1.00 O3G C:GTP601 1.8 0.2 1.0 O2G C:GTP601 2.0 0.1 1.0 PG C:GTP601 2.2 0.6 1.0 O2B C:GTP601 2.5 1.0 1.0 O3B C:GTP601 3.0 0.9 1.0 PB C:GTP601 3.1 0.7 1.0 CA C:GLY144 3.2 0.6 1.0 N C:THR145 3.2 0.7 1.0 N C:GLY144 3.3 0.6 1.0 CG2 C:THR145 3.6 0.8 1.0 O1G C:GTP601 3.6 0.8 1.0 O3A C:GTP601 3.7 0.5 1.0 C C:GLY144 3.7 0.7 1.0 N C:ASN101 3.8 0.3 1.0 C C:GLY143 4.1 0.5 1.0 CB C:ALA100 4.1 0.2 1.0 C C:ALA99 4.1 0.4 1.0 OG1 C:THR145 4.1 0.8 1.0 N C:ALA100 4.2 0.5 1.0 O C:ALA99 4.3 0.4 1.0 CB C:THR145 4.3 0.8 1.0 CA C:THR145 4.3 0.7 1.0 ND2 C:ASN101 4.3 0.3 1.0 CB C:ALA99 4.4 0.4 1.0 CA C:ASN101 4.4 0.2 1.0 CA C:ALA100 4.5 0.4 1.0 C C:ALA100 4.5 0.3 1.0 O1B C:GTP601 4.5 0.1 1.0 O C:GLY143 4.5 0.4 1.0 CG C:ASN101 4.6 0.2 1.0 CA C:ALA99 4.7 0.4 1.0 OD1 C:ASN101 4.8 0.1 1.0 CA C:GLY143 4.9 0.5 1.0 O C:GLY144 5.0 0.7 1.0

R.B.Ravelli, B.Gigant, P.A.Curmi, I.Jourdain, S.Lachkar, A.Sobel, M.Knossow. Insight Into Tubulin Regulation From A Complex with Colchicine and A Stathmin-Like Domain. Nature V. 428 198 2004.
ISSN: ISSN 0028-0836
PubMed: 15014504
DOI: 10.1038/NATURE02393