Magnesium in PDB 4pj3: Structural Insight Into the Function and Evolution of the Spliceosomal Helicase Aquarius, Structure of Aquarius in Complex with Amppnp

The structure of Structural Insight Into the Function and Evolution of the Spliceosomal Helicase Aquarius, Structure of Aquarius in Complex with Amppnp, PDB code: 4pj3 was solved by I.De, S.Bessonov, R.Hofele, K.F.Dos Santos, C.L.Will, H.Urlaub, R.Luhrmann, V.Pena, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	47.49 / 2.30
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	94.986, 140.444, 144.761, 90.00, 90.00, 90.00
R / Rfree (%)	19.1 / 22.6

The binding sites of Magnesium atom in the Structural Insight Into the Function and Evolution of the Spliceosomal Helicase Aquarius, Structure of Aquarius in Complex with Amppnp (pdb code 4pj3). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the Structural Insight Into the Function and Evolution of the Spliceosomal Helicase Aquarius, Structure of Aquarius in Complex with Amppnp, PDB code: 4pj3:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the Structural Insight Into the Function and Evolution of the Spliceosomal Helicase Aquarius, Structure of Aquarius in Complex with Amppnp


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Structural Insight Into the Function and Evolution of the Spliceosomal Helicase Aquarius, Structure of Aquarius in Complex with Amppnp within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg1502 b:47.8 occ:1.00 N3B A:ANP1501 2.7 28.2 1.0 OE1 A:GLN861 2.8 30.8 1.0 OG1 A:THR830 3.1 29.4 1.0 O A:HOH1821 3.4 31.7 1.0 O1B A:ANP1501 3.7 28.1 1.0 PB A:ANP1501 3.8 30.2 1.0 O2G A:ANP1501 3.8 39.7 1.0 CD A:GLN861 3.8 36.4 1.0 PG A:ANP1501 3.9 32.4 1.0 NE2 A:GLN861 4.3 35.6 1.0 CB A:THR830 4.5 28.6 1.0 O2B A:ANP1501 4.6 24.2 1.0 CE A:LYS829 4.7 26.0 1.0 O1G A:ANP1501 4.9 42.1 1.0 O3G A:ANP1501 4.9 30.9 1.0

Magnesium binding site 2 out of 2 in the Structural Insight Into the Function and Evolution of the Spliceosomal Helicase Aquarius, Structure of Aquarius in Complex with Amppnp


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Structural Insight Into the Function and Evolution of the Spliceosomal Helicase Aquarius, Structure of Aquarius in Complex with Amppnp within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg1503 b:66.3 occ:1.00 O A:HOH1855 3.6 36.5 1.0 N A:ASP160 4.0 32.6 1.0 CE A:LYS113 4.1 34.4 1.0 CA A:VAL159 4.2 30.7 1.0 OD2 A:ASP160 4.2 47.9 1.0 O A:GLU158 4.3 35.4 1.0 CG A:ASP160 4.3 39.8 1.0 CG1 A:VAL159 4.4 29.0 1.0 OD1 A:ASP160 4.5 35.6 1.0 NZ A:LYS113 4.6 44.3 1.0 C A:VAL159 4.6 34.0 1.0 CB A:ASP160 4.7 28.5 1.0 CB A:VAL159 4.8 30.7 1.0 CA A:ASP160 5.0 29.5 1.0

I.De, S.Bessonov, R.Hofele, K.Dos Santos, C.L.Will, H.Urlaub, R.Luhrmann, V.Pena. The Rna Helicase Aquarius Exhibits Structural Adaptations Mediating Its Recruitment to Spliceosomes. Nat.Struct.Mol.Biol. 2015.
ISSN: ESSN 1545-9985
PubMed: 25599396
DOI: 10.1038/NSMB.2951