Magnesium in PDB 6flq: Cryoem Structure of E.Coli Rna Polymerase Paused Elongation Complex Bound to Nusa

All present enzymatic activity of Cryoem Structure of E.Coli Rna Polymerase Paused Elongation Complex Bound to Nusa:
2.7.7.6;

The structure of Cryoem Structure of E.Coli Rna Polymerase Paused Elongation Complex Bound to Nusa also contains other interesting chemical elements:

Zinc

(Zn)

2 atoms

The binding sites of Magnesium atom in the Cryoem Structure of E.Coli Rna Polymerase Paused Elongation Complex Bound to Nusa (pdb code 6flq). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total only one binding site of Magnesium was determined in the Cryoem Structure of E.Coli Rna Polymerase Paused Elongation Complex Bound to Nusa, PDB code: 6flq:

Magnesium binding site 1 out of 1 in the Cryoem Structure of E.Coli Rna Polymerase Paused Elongation Complex Bound to Nusa


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Cryoem Structure of E.Coli Rna Polymerase Paused Elongation Complex Bound to Nusa within 5.0Å range: probe atom residue distance (Å) B Occ D:Mg1501 b:57.9 occ:1.00 OD1 D:ASP462 2.5 42.5 1.0 OD1 D:ASP464 2.8 41.4 1.0 CG D:ASP462 3.2 42.5 1.0 OD2 D:ASP462 3.2 42.5 1.0 C5' R:U29 3.3 69.8 1.0 CG D:ASP464 3.6 41.4 1.0 OD2 D:ASP464 3.6 41.4 1.0 C3' R:U29 3.9 69.8 1.0 C4' R:U29 4.1 69.8 1.0 O5' R:U29 4.4 69.8 1.0 NH2 D:ARG425 4.6 44.9 1.0 OP1 R:U29 4.6 69.8 1.0 CB D:ASP460 4.7 43.1 1.0 CB D:ASP462 4.7 42.5 1.0 C2' R:U29 4.8 69.8 1.0 OD2 D:ASP460 4.9 43.1 1.0 O3' R:U29 4.9 69.8 1.0

X.Guo, A.G.Myasnikov, J.Chen, C.Crucifix, G.Papai, M.Takacs, P.Schultz, A.Weixlbaumer. Structural Basis For Nusa Stabilized Transcriptional Pausing. Mol. Cell V. 69 816 2018.
ISSN: ISSN 1097-4164
PubMed: 29499136
DOI: 10.1016/J.MOLCEL.2018.02.008