Magnesium in PDB 6j7p: Crystal Structure of Toxin Tglt (Unusual Type Guanylyltransferase-Like Toxin, RV1045) Mutant E146Q Co-Expressed with Taka From Mycobacterium Tuberculosis

The structure of Crystal Structure of Toxin Tglt (Unusual Type Guanylyltransferase-Like Toxin, RV1045) Mutant E146Q Co-Expressed with Taka From Mycobacterium Tuberculosis, PDB code: 6j7p was solved by X.Yu, X.Gao, K.Zhu, J.A.Wojdyla, M.Wang, S.Cui, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	47.34 / 2.63
Space group	P 32 2 1
Cell size a, b, c (Å), α, β, γ (°)	94.691, 94.691, 67.252, 90.00, 90.00, 120.00
R / Rfree (%)	27.2 / 29.5

The binding sites of Magnesium atom in the Crystal Structure of Toxin Tglt (Unusual Type Guanylyltransferase-Like Toxin, RV1045) Mutant E146Q Co-Expressed with Taka From Mycobacterium Tuberculosis (pdb code 6j7p). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 3 binding sites of Magnesium where determined in the Crystal Structure of Toxin Tglt (Unusual Type Guanylyltransferase-Like Toxin, RV1045) Mutant E146Q Co-Expressed with Taka From Mycobacterium Tuberculosis, PDB code: 6j7p:
Jump to Magnesium binding site number: 1; 2; 3;

Magnesium binding site 1 out of 3 in the Crystal Structure of Toxin Tglt (Unusual Type Guanylyltransferase-Like Toxin, RV1045) Mutant E146Q Co-Expressed with Taka From Mycobacterium Tuberculosis


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Crystal Structure of Toxin Tglt (Unusual Type Guanylyltransferase-Like Toxin, RV1045) Mutant E146Q Co-Expressed with Taka From Mycobacterium Tuberculosis within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg301 b:56.6 occ:0.50 HB A:THR249 1.8 61.6 1.0 CB A:THR249 2.6 51.3 1.0 HG22 A:THR249 2.6 65.4 1.0 HG21 A:THR249 2.7 65.4 1.0 CG2 A:THR249 2.8 54.5 1.0 HG1 A:THR249 3.1 62.0 1.0 OD1 A:ASP274 3.1 53.4 1.0 OG1 A:THR249 3.3 51.6 1.0 HG23 A:THR249 3.7 65.4 1.0 CA A:THR249 3.8 50.1 1.0 H A:THR249 3.8 62.0 1.0 O A:THR249 4.0 58.0 1.0 C A:THR249 4.1 52.5 1.0 CG A:ASP274 4.1 47.9 1.0 N A:THR249 4.2 51.6 1.0 HA A:THR249 4.5 60.1 1.0 OD2 A:ASP274 4.5 49.4 1.0 HA A:ASP274 4.6 55.5 1.0 HG13 A:VAL273 4.6 60.0 1.0 N A:LEU250 5.0 49.6 1.0

Magnesium binding site 2 out of 3 in the Crystal Structure of Toxin Tglt (Unusual Type Guanylyltransferase-Like Toxin, RV1045) Mutant E146Q Co-Expressed with Taka From Mycobacterium Tuberculosis


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Crystal Structure of Toxin Tglt (Unusual Type Guanylyltransferase-Like Toxin, RV1045) Mutant E146Q Co-Expressed with Taka From Mycobacterium Tuberculosis within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg302 b:61.2 occ:1.00 HD3 A:ARG205 2.4 83.4 1.0 HD2 A:ARG205 2.8 83.4 1.0 O A:ALA192 2.9 52.6 1.0 CD A:ARG205 3.0 69.5 1.0 HB3 A:ALA195 3.0 66.6 1.0 HB1 A:ALA192 3.2 62.3 1.0 HG3 A:ARG205 3.4 78.5 1.0 HA A:ALA192 3.4 61.3 1.0 OD1 A:ASN203 3.5 57.7 1.0 HD21 A:LEU197 3.6 83.4 1.0 C A:ALA192 3.7 51.7 1.0 CG A:ARG205 3.8 65.4 1.0 CB A:ALA195 3.8 55.5 1.0 CA A:ALA192 3.9 51.1 1.0 HB2 A:ALA195 3.9 66.6 1.0 CB A:ALA192 3.9 51.9 1.0 CG A:ASN203 4.0 56.7 1.0 HD11 A:LEU197 4.1 93.6 1.0 HD21 A:ASN203 4.1 64.5 1.0 NE A:ARG205 4.2 70.1 1.0 HB1 A:ALA195 4.2 66.6 1.0 HB2 A:ARG205 4.2 77.0 1.0 ND2 A:ASN203 4.3 53.8 1.0 HB2 A:ALA192 4.4 62.3 1.0 HE A:ARG205 4.4 84.1 1.0 HG2 A:ARG205 4.5 78.5 1.0 CD2 A:LEU197 4.5 69.5 1.0 H A:ALA195 4.6 64.6 1.0 CB A:ARG205 4.6 64.2 1.0 HB3 A:ALA192 4.6 62.3 1.0 HA A:ASN203 4.7 73.0 1.0 HB2 A:ASN203 4.7 72.3 1.0 HD23 A:LEU197 4.8 83.4 1.0 HG A:LEU197 4.8 85.5 1.0 HH11 A:ARG205 4.8 0.9 1.0 HD22 A:ASN203 4.9 64.5 1.0 N A:VAL193 4.9 51.7 1.0 HA A:VAL193 4.9 62.4 1.0 CB A:ASN203 4.9 60.3 1.0 CD1 A:LEU197 4.9 78.0 1.0 HB3 A:ARG205 5.0 77.0 1.0

Magnesium binding site 3 out of 3 in the Crystal Structure of Toxin Tglt (Unusual Type Guanylyltransferase-Like Toxin, RV1045) Mutant E146Q Co-Expressed with Taka From Mycobacterium Tuberculosis


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 3 of Crystal Structure of Toxin Tglt (Unusual Type Guanylyltransferase-Like Toxin, RV1045) Mutant E146Q Co-Expressed with Taka From Mycobacterium Tuberculosis within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg303 b:50.4 occ:1.00 HG2 A:GLU216 1.9 60.0 1.0 O A:HOH407 2.0 44.3 1.0 CG A:GLU216 2.7 50.0 1.0 HG3 A:GLU216 2.9 60.0 1.0 CD A:GLU216 3.4 52.1 1.0 O A:GLN213 3.7 46.2 1.0 OE1 A:GLU216 3.8 52.0 1.0 HB3 A:GLU216 3.8 56.9 1.0 CB A:GLU216 3.9 47.4 1.0 H A:GLY217 4.0 61.3 1.0 HA A:GLN213 4.1 55.0 1.0 N A:GLY217 4.1 51.0 1.0 HA2 A:GLY217 4.1 59.5 1.0 OE2 A:GLU216 4.1 58.1 1.0 HB3 A:GLN213 4.3 54.8 1.0 C A:GLU216 4.5 51.2 1.0 CA A:GLY217 4.5 49.6 1.0 HA3 A:GLY217 4.5 59.5 1.0 HB2 A:GLU216 4.6 56.9 1.0 C A:GLN213 4.6 46.3 1.0 CA A:GLN213 4.7 45.8 1.0 CA A:GLU216 4.8 49.4 1.0 O A:GLU216 5.0 51.7 1.0

X.Yu, X.Gao, K.Zhu, H.Yin, X.Mao, J.A.Wojdyla, B.Qin, H.Huang, M.Wang, Y.Sun, S.Cui. Characterization of A Toxin-Antitoxin System in Mycobacterium Tuberculosis Suggests Neutralization By Phosphorylation As the Antitoxicity Mechanism Commun Biol 2020.
ISSN: ESSN 2399-3642