Magnesium in PDB 6jcw: Cryo-Em Structure of Sulfolobus Solfataricus Ketol-Acid Reductoisomerase (Sso-Kari) with MG2+ at PH8.5

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>>> Page 1 <<< Page 2, Binding sites: 11 - 20; Page 3, Binding sites: 21 - 24;

Binding sites:

The binding sites of Magnesium atom in the Cryo-Em Structure of Sulfolobus Solfataricus Ketol-Acid Reductoisomerase (Sso-Kari) with MG2+ at PH8.5 (pdb code 6jcw). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 24 binding sites of Magnesium where determined in the Cryo-Em Structure of Sulfolobus Solfataricus Ketol-Acid Reductoisomerase (Sso-Kari) with MG2+ at PH8.5, PDB code: 6jcw:
Jump to Magnesium binding site number: 1; 2; 3; 4; 5; 6; 7; 8; 9; 10;

Magnesium binding site 1 out of 24 in the Cryo-Em Structure of Sulfolobus Solfataricus Ketol-Acid Reductoisomerase (Sso-Kari) with MG2+ at PH8.5


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Cryo-Em Structure of Sulfolobus Solfataricus Ketol-Acid Reductoisomerase (Sso-Kari) with MG2+ at PH8.5 within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg401 b:70.5 occ:1.00 OD1 A:ASP191 3.4 68.5 1.0 OD2 A:ASP191 3.5 68.5 1.0 CG A:ASP191 3.9 68.5 1.0 OE2 A:GLU195 4.0 63.6 1.0 OG B:SER257 4.4 64.3 1.0

Magnesium binding site 2 out of 24 in the Cryo-Em Structure of Sulfolobus Solfataricus Ketol-Acid Reductoisomerase (Sso-Kari) with MG2+ at PH8.5


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of Cryo-Em Structure of Sulfolobus Solfataricus Ketol-Acid Reductoisomerase (Sso-Kari) with MG2+ at PH8.5 within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg402 b:72.1 occ:1.00 OE1 A:GLU227 2.2 78.1 1.0 OE2 A:GLU233 3.1 96.2 1.0 CD A:GLU227 3.2 78.1 1.0 OE1 A:GLU233 3.5 96.2 1.0 OE2 A:GLU227 3.6 78.1 1.0 CD A:GLU233 3.6 96.2 1.0 OG B:SER194 3.7 65.1 1.0 CB B:SER194 4.0 65.1 1.0 OD1 B:ASP191 4.2 68.5 1.0 O A:GLU227 4.4 78.1 1.0 CG A:GLU227 4.6 78.1 1.0 O B:ASP191 4.9 68.5 1.0 NH1 B:ARG130 4.9 93.6 1.0

Magnesium binding site 3 out of 24 in the Cryo-Em Structure of Sulfolobus Solfataricus Ketol-Acid Reductoisomerase (Sso-Kari) with MG2+ at PH8.5


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 3 of Cryo-Em Structure of Sulfolobus Solfataricus Ketol-Acid Reductoisomerase (Sso-Kari) with MG2+ at PH8.5 within 5.0Å range: probe atom residue distance (Å) B Occ B:Mg401 b:71.3 occ:1.00 OE1 B:GLU227 2.2 78.2 1.0 OE2 B:GLU233 2.7 95.0 1.0 CD B:GLU227 3.2 78.2 1.0 OE2 B:GLU227 3.6 78.2 1.0 OG A:SER194 3.7 65.2 1.0 CD B:GLU233 3.9 95.0 1.0 CB A:SER194 4.0 65.2 1.0 OD1 A:ASP191 4.2 68.5 1.0 O B:GLU227 4.4 78.2 1.0 CG B:GLU227 4.5 78.2 1.0 OE1 B:GLU233 4.5 95.0 1.0 O A:ASP191 4.9 68.5 1.0 NH1 A:ARG130 4.9 94.6 1.0 CG B:GLU233 5.0 95.0 1.0

Magnesium binding site 4 out of 24 in the Cryo-Em Structure of Sulfolobus Solfataricus Ketol-Acid Reductoisomerase (Sso-Kari) with MG2+ at PH8.5


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 4 of Cryo-Em Structure of Sulfolobus Solfataricus Ketol-Acid Reductoisomerase (Sso-Kari) with MG2+ at PH8.5 within 5.0Å range: probe atom residue distance (Å) B Occ B:Mg402 b:71.1 occ:1.00 OD1 B:ASP191 3.4 68.5 1.0 OD2 B:ASP191 3.5 68.5 1.0 CG B:ASP191 3.9 68.5 1.0 OE2 B:GLU195 4.0 63.3 1.0 OG A:SER257 4.4 64.2 1.0

Magnesium binding site 5 out of 24 in the Cryo-Em Structure of Sulfolobus Solfataricus Ketol-Acid Reductoisomerase (Sso-Kari) with MG2+ at PH8.5


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 5 of Cryo-Em Structure of Sulfolobus Solfataricus Ketol-Acid Reductoisomerase (Sso-Kari) with MG2+ at PH8.5 within 5.0Å range: probe atom residue distance (Å) B Occ C:Mg401 b:71.5 occ:1.00 OD1 C:ASP191 3.4 68.6 1.0 OD2 C:ASP191 3.5 68.6 1.0 CG C:ASP191 3.9 68.6 1.0 OE2 C:GLU195 4.0 63.4 1.0 OG D:SER257 4.4 64.1 1.0

Magnesium binding site 6 out of 24 in the Cryo-Em Structure of Sulfolobus Solfataricus Ketol-Acid Reductoisomerase (Sso-Kari) with MG2+ at PH8.5


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 6 of Cryo-Em Structure of Sulfolobus Solfataricus Ketol-Acid Reductoisomerase (Sso-Kari) with MG2+ at PH8.5 within 5.0Å range: probe atom residue distance (Å) B Occ C:Mg402 b:70.6 occ:1.00 OE1 C:GLU227 2.2 77.8 1.0 OE2 C:GLU233 3.1 95.4 1.0 CD C:GLU227 3.2 77.8 1.0 OE1 C:GLU233 3.5 95.4 1.0 OE2 C:GLU227 3.6 77.8 1.0 CD C:GLU233 3.6 95.4 1.0 OG D:SER194 3.7 65.0 1.0 CB D:SER194 4.0 65.0 1.0 OD1 D:ASP191 4.2 68.1 1.0 O C:GLU227 4.4 77.8 1.0 CG C:GLU227 4.6 77.8 1.0 O D:ASP191 4.9 68.1 1.0 NH1 D:ARG130 4.9 93.1 1.0

Magnesium binding site 7 out of 24 in the Cryo-Em Structure of Sulfolobus Solfataricus Ketol-Acid Reductoisomerase (Sso-Kari) with MG2+ at PH8.5


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 7 of Cryo-Em Structure of Sulfolobus Solfataricus Ketol-Acid Reductoisomerase (Sso-Kari) with MG2+ at PH8.5 within 5.0Å range: probe atom residue distance (Å) B Occ D:Mg401 b:70.7 occ:1.00 OE1 D:GLU227 2.2 77.9 1.0 OE2 D:GLU233 3.1 96.2 1.0 CD D:GLU227 3.2 77.9 1.0 OE1 D:GLU233 3.5 96.2 1.0 OE2 D:GLU227 3.6 77.9 1.0 CD D:GLU233 3.6 96.2 1.0 OG C:SER194 3.7 65.3 1.0 CB C:SER194 4.0 65.3 1.0 OD1 C:ASP191 4.2 68.6 1.0 O D:GLU227 4.4 77.9 1.0 CG D:GLU227 4.6 77.9 1.0 O C:ASP191 4.9 68.6 1.0 NH1 C:ARG130 4.9 94.3 1.0

Magnesium binding site 8 out of 24 in the Cryo-Em Structure of Sulfolobus Solfataricus Ketol-Acid Reductoisomerase (Sso-Kari) with MG2+ at PH8.5


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 8 of Cryo-Em Structure of Sulfolobus Solfataricus Ketol-Acid Reductoisomerase (Sso-Kari) with MG2+ at PH8.5 within 5.0Å range: probe atom residue distance (Å) B Occ D:Mg402 b:70.1 occ:1.00 OD1 D:ASP191 3.4 68.1 1.0 OD2 D:ASP191 3.5 68.1 1.0 CG D:ASP191 3.9 68.1 1.0 OE2 D:GLU195 4.0 63.1 1.0 OG C:SER257 4.4 64.2 1.0

Magnesium binding site 9 out of 24 in the Cryo-Em Structure of Sulfolobus Solfataricus Ketol-Acid Reductoisomerase (Sso-Kari) with MG2+ at PH8.5


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 9 of Cryo-Em Structure of Sulfolobus Solfataricus Ketol-Acid Reductoisomerase (Sso-Kari) with MG2+ at PH8.5 within 5.0Å range: probe atom residue distance (Å) B Occ E:Mg401 b:72.1 occ:1.00 OD1 E:ASP191 3.4 68.5 1.0 OD2 E:ASP191 3.5 68.5 1.0 CG E:ASP191 3.9 68.5 1.0 OE2 E:GLU195 4.0 63.2 1.0 OG F:SER257 4.3 64.2 1.0

Magnesium binding site 10 out of 24 in the Cryo-Em Structure of Sulfolobus Solfataricus Ketol-Acid Reductoisomerase (Sso-Kari) with MG2+ at PH8.5


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 10 of Cryo-Em Structure of Sulfolobus Solfataricus Ketol-Acid Reductoisomerase (Sso-Kari) with MG2+ at PH8.5 within 5.0Å range: probe atom residue distance (Å) B Occ E:Mg402 b:71.0 occ:1.00 OE1 E:GLU227 2.2 78.4 1.0 OE2 E:GLU233 2.7 95.2 1.0 CD E:GLU227 3.2 78.4 1.0 OE2 E:GLU227 3.6 78.4 1.0 OG F:SER194 3.7 65.2 1.0 CD E:GLU233 3.9 95.2 1.0 CB F:SER194 4.0 65.2 1.0 OD1 F:ASP191 4.2 68.6 1.0 O E:GLU227 4.4 78.4 1.0 OE1 E:GLU233 4.5 95.2 1.0 CG E:GLU227 4.5 78.4 1.0 O F:ASP191 4.9 68.6 1.0 NH1 F:ARG130 4.9 94.0 1.0 CG E:GLU233 5.0 95.2 1.0

C.Y.Chen, Y.C.Chang, B.L.Lin, K.F.Lin, C.H.Huang, D.L.Hsieh, T.P.Ko, M.D.Tsai. Use of Cryo-Em to Uncover Structural Bases of pH Effect and Cofactor Bispecificity of Ketol-Acid Reductoisomerase. J. Am. Chem. Soc. V. 141 6136 2019.
ISSN: ESSN 1520-5126
PubMed: 30921515
DOI: 10.1021/JACS.9B01354