Magnesium in PDB 8eh9: Cryo-Em Structure of His-Elemental Paused Elongation Complex with A Folded Tl and A Rotated Rh-Fl (2)

Enzymatic activity of Cryo-Em Structure of His-Elemental Paused Elongation Complex with A Folded Tl and A Rotated Rh-Fl (2)

All present enzymatic activity of Cryo-Em Structure of His-Elemental Paused Elongation Complex with A Folded Tl and A Rotated Rh-Fl (2):
2.7.7.6;

Other elements in 8eh9:

The structure of Cryo-Em Structure of His-Elemental Paused Elongation Complex with A Folded Tl and A Rotated Rh-Fl (2) also contains other interesting chemical elements:

Zinc

(Zn)

2 atoms

Magnesium Binding Sites:

The binding sites of Magnesium atom in the Cryo-Em Structure of His-Elemental Paused Elongation Complex with A Folded Tl and A Rotated Rh-Fl (2) (pdb code 8eh9). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total only one binding site of Magnesium was determined in the Cryo-Em Structure of His-Elemental Paused Elongation Complex with A Folded Tl and A Rotated Rh-Fl (2), PDB code: 8eh9:

Magnesium binding site 1 out of 1 in 8eh9

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Magnesium Binding Sites List in 8eh9

Magnesium binding site 1 out of 1 in the Cryo-Em Structure of His-Elemental Paused Elongation Complex with A Folded Tl and A Rotated Rh-Fl (2)

Mono view

Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Cryo-Em Structure of His-Elemental Paused Elongation Complex with A Folded Tl and A Rotated Rh-Fl (2) within 5.0Å range:

probe	atom	residue	distance (Å)	B	Occ
R:Mg101 b:21.9 occ:1.00	O3'	R:C18	2.0	18.4	1.0
	OD1	J:ASP460	2.1	16.3	1.0
	O5'	R:U19	2.2	23.3	1.0
	OD2	J:ASP460	2.2	16.3	1.0
	CG	J:ASP460	2.4	16.3	1.0
	P	R:U19	2.6	23.3	1.0
	O2'	R:C18	3.0	18.4	1.0
	C3'	R:C18	3.2	18.4	1.0
	OP1	R:U19	3.2	23.3	1.0
	C5'	R:U19	3.3	23.3	1.0
	NH2	J:ARG425	3.5	15.4	1.0
	OD1	J:ASP462	3.5	17.6	1.0
	C2'	R:C18	3.6	18.4	1.0
	C4'	R:U19	3.6	23.3	1.0
	O4'	R:U19	3.7	23.3	1.0
	OD2	J:ASP462	3.7	17.6	1.0
	OP2	R:U19	3.8	23.3	1.0
	CB	J:ASP460	3.9	16.3	1.0
	CG	J:ASP462	4.0	17.6	1.0
	C4'	R:C18	4.1	18.4	1.0
	N	J:ASP460	4.4	16.3	1.0
	CA	J:ASP460	4.7	16.3	1.0
	CZ	J:ARG425	4.7	15.4	1.0
	O	J:ASN458	4.8	16.8	1.0
	O	J:ASP460	4.9	16.3	1.0
	OD2	J:ASP464	4.9	14.6	1.0
	C1'	R:C18	4.9	18.4	1.0
	C1'	R:U19	5.0	23.3	1.0
	C5'	R:C18	5.0	18.4	1.0

Reference:

J.Y.Kang, T.V.Mishanina, Y.Bao, J.Chen, E.Llewellyn, J.Liu, S.A.Darst, R.Landick. An Ensemble of Interconverting Conformations of the Elemental Paused Transcription Complex Creates Regulatory Options. Proc.Natl.Acad.Sci.Usa V. 120 45120 2023.
ISSN: ESSN 1091-6490
PubMed: 36795753
DOI: 10.1073/PNAS.2215945120

Page generated: Fri Oct 4 01:14:12 2024

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