Magnesium in PDB 8ej3: M. Tuberculosis Rnap Pause Escaped Complex with Bacillus Subtilis Nusg and Gmpcpp

All present enzymatic activity of M. Tuberculosis Rnap Pause Escaped Complex with Bacillus Subtilis Nusg and Gmpcpp:
2.7.7.6;

The structure of M. Tuberculosis Rnap Pause Escaped Complex with Bacillus Subtilis Nusg and Gmpcpp also contains other interesting chemical elements:

Zinc

(Zn)

2 atoms

The binding sites of Magnesium atom in the M. Tuberculosis Rnap Pause Escaped Complex with Bacillus Subtilis Nusg and Gmpcpp (pdb code 8ej3). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total 2 binding sites of Magnesium where determined in the M. Tuberculosis Rnap Pause Escaped Complex with Bacillus Subtilis Nusg and Gmpcpp, PDB code: 8ej3:
Jump to Magnesium binding site number: 1; 2;

Magnesium binding site 1 out of 2 in the M. Tuberculosis Rnap Pause Escaped Complex with Bacillus Subtilis Nusg and Gmpcpp


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of M. Tuberculosis Rnap Pause Escaped Complex with Bacillus Subtilis Nusg and Gmpcpp within 5.0Å range: probe atom residue distance (Å) B Occ D:Mg2001 b:41.1 occ:1.00 OD2 D:ASP535 2.1 58.3 1.0 OD1 D:ASP535 2.2 56.1 1.0 OD1 D:ASP537 2.3 43.6 1.0 O3' R:A30 2.3 51.5 1.0 CG D:ASP535 2.4 58.4 1.0 OD2 D:ASP537 2.9 55.1 1.0 CG D:ASP537 2.9 40.9 1.0 OD1 D:ASP539 3.1 51.8 1.0 C3' R:A30 3.7 44.4 1.0 CB D:ASP535 3.8 39.8 1.0 CG D:ASP539 4.0 50.8 1.0 OD2 D:ASP539 4.1 53.8 1.0 O2' R:A30 4.3 67.0 1.0 O D:ASP535 4.4 40.9 1.0 CB D:ASP537 4.4 31.6 1.0 C4' R:A30 4.4 48.0 1.0 C2' R:A30 4.6 50.0 1.0 C D:ASP535 4.6 44.5 1.0 CA D:ASP535 4.6 37.7 1.0 N D:ASP537 4.7 48.5 1.0 N D:ASP535 4.8 46.4 1.0 O5' C:G2P1201 4.8 87.2 1.0 C5' R:A30 4.9 46.7 1.0 O2A C:G2P1201 5.0 82.2 1.0 CA D:ASP537 5.0 39.1 1.0

Magnesium binding site 2 out of 2 in the M. Tuberculosis Rnap Pause Escaped Complex with Bacillus Subtilis Nusg and Gmpcpp


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 2 of M. Tuberculosis Rnap Pause Escaped Complex with Bacillus Subtilis Nusg and Gmpcpp within 5.0Å range: probe atom residue distance (Å) B Occ D:Mg2002 b:65.4 occ:1.00 O1B C:G2P1201 2.0 92.1 1.0 OD2 D:ASP535 3.3 58.3 1.0 O2A C:G2P1201 3.5 82.2 1.0 O2G C:G2P1201 3.5 85.2 1.0 PB C:G2P1201 3.6 102.2 1.0 CB D:ASP535 3.6 39.8 1.0 CG D:ASP535 3.7 58.4 1.0 O2B C:G2P1201 4.3 88.3 1.0 PG C:G2P1201 4.4 89.5 1.0 OE2 C:GLU732 4.4 56.3 1.0 O3B C:G2P1201 4.5 88.4 1.0 PA C:G2P1201 4.5 96.2 1.0 C3A C:G2P1201 4.5 83.3 1.0 O1G C:G2P1201 4.5 78.9 1.0 OD1 D:ASP535 4.7 56.1 1.0 OD2 C:ASP733 4.7 52.3 1.0 OE1 C:GLU732 4.7 58.3 1.0 O5' C:G2P1201 4.8 87.2 1.0 CD C:GLU732 4.9 54.0 1.0 CA D:ASP535 5.0 37.7 1.0

R.K.Vishwakarma, M.Z.Qayyum, P.Babitzke, K.S.Murakami. Structural Basis of Nusg-Dependent Rna Polymerase Transcription Pausing in Bacteria To Be Published.