Magnesium in PDB 5ui8: Structure of Sigman-Holoenzyme

All present enzymatic activity of Structure of Sigman-Holoenzyme:
2.7.7.6;

The structure of Structure of Sigman-Holoenzyme, PDB code: 5ui8 was solved by S.A.Darst, E.A.Campbell, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	29.64 / 3.76
Space group	P 21 21 2
Cell size a, b, c (Å), α, β, γ (°)	208.476, 151.524, 195.279, 90.00, 90.00, 90.00
R / Rfree (%)	26.2 / 31.2

The structure of Structure of Sigman-Holoenzyme also contains other interesting chemical elements:

Zinc

(Zn)

2 atoms

The binding sites of Magnesium atom in the Structure of Sigman-Holoenzyme (pdb code 5ui8). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total only one binding site of Magnesium was determined in the Structure of Sigman-Holoenzyme, PDB code: 5ui8:

Magnesium binding site 1 out of 1 in the Structure of Sigman-Holoenzyme


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Structure of Sigman-Holoenzyme within 5.0Å range: probe atom residue distance (Å) B Occ J:Mg1501 b:0.5 occ:1.00 OD1 J:ASP462 2.8 0.1 1.0 OD2 J:ASP460 3.1 0.1 1.0 OD2 J:ASP462 3.1 0.8 1.0 CB J:ASP460 3.2 0.4 1.0 CG J:ASP462 3.3 0.5 1.0 CG J:ASP460 3.6 0.2 1.0 OD2 J:ASP464 3.7 0.1 1.0 OD1 J:ASP464 4.2 0.6 1.0 CG J:ASP464 4.3 0.3 1.0 CA J:ASP460 4.4 0.5 1.0 NH2 J:ARG425 4.5 0.7 1.0 N J:ASP460 4.6 0.8 1.0 CB J:ASP462 4.8 0.9 1.0 OD1 J:ASP460 4.8 0.8 1.0 C J:ASP460 5.0 0.1 1.0

E.A.Campbell, S.Kamath, K.R.Rajashankar, M.Wu, S.A.Darst. Crystal Structure of Aquifex Aeolicus Sigma (N) Bound to Promoter Dna and the Structure of Sigma (N)-Holoenzyme. Proc. Natl. Acad. Sci. V. 114 E1805 2017U.S.A..
ISSN: ESSN 1091-6490
PubMed: 28223493
DOI: 10.1073/PNAS.1619464114