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Magnesium in PDB 5vu7: Tna Polymerase, Open Ternary Complex

Enzymatic activity of Tna Polymerase, Open Ternary Complex

All present enzymatic activity of Tna Polymerase, Open Ternary Complex:
2.7.7.7;

Protein crystallography data

The structure of Tna Polymerase, Open Ternary Complex, PDB code: 5vu7 was solved by N.Chim, J.C.Chaput, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 49.10 / 2.72
Space group P 2 21 21
Cell size a, b, c (Å), α, β, γ (°) 66.550, 112.610, 145.460, 90.00, 90.00, 90.00
R / Rfree (%) 21.4 / 26.8

Magnesium Binding Sites:

The binding sites of Magnesium atom in the Tna Polymerase, Open Ternary Complex (pdb code 5vu7). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total only one binding site of Magnesium was determined in the Tna Polymerase, Open Ternary Complex, PDB code: 5vu7:

Magnesium binding site 1 out of 1 in 5vu7

Go back to Magnesium Binding Sites List in 5vu7
Magnesium binding site 1 out of 1 in the Tna Polymerase, Open Ternary Complex


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of Tna Polymerase, Open Ternary Complex within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Mg801

b:44.6
occ:1.00
OH A:TYR583 3.5 69.8 1.0
OE2 A:GLU580 3.9 46.6 1.0
OE1 A:GLU580 4.0 56.1 1.0
CD A:GLU580 4.1 52.7 1.0
CZ A:TYR583 4.3 63.1 1.0
CE1 A:TYR583 4.7 62.9 1.0
NH1 A:ARG606 4.8 39.7 1.0
CZ A:PHE545 4.9 47.4 1.0
CG2 A:THR604 5.0 59.5 1.0
NE A:ARG606 5.0 42.0 1.0

Reference:

N.Chim, C.Shi, S.P.Sau, A.Nikoomanzar, J.C.Chaput. Structural Basis For Tna Synthesis By An Engineered Tna Polymerase. Nat Commun V. 8 1810 2017.
ISSN: ESSN 2041-1723
PubMed: 29180809
DOI: 10.1038/S41467-017-02014-0
Page generated: Mon Sep 30 06:20:47 2024

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