Magnesium in PDB 9eak: SPCAS9 with 17-Bp R-Loop Containing 2 Terminal Mismatches (State I - Pre-Activation)

The binding sites of Magnesium atom in the SPCAS9 with 17-Bp R-Loop Containing 2 Terminal Mismatches (State I - Pre-Activation) (pdb code 9eak). This binding sites where shown within 5.0 Angstroms radius around Magnesium atom.
In total only one binding site of Magnesium was determined in the SPCAS9 with 17-Bp R-Loop Containing 2 Terminal Mismatches (State I - Pre-Activation), PDB code: 9eak:

Magnesium binding site 1 out of 1 in the SPCAS9 with 17-Bp R-Loop Containing 2 Terminal Mismatches (State I - Pre-Activation)


Mono view


Stereo pair view

A full contact list of Magnesium with other atoms in the Mg binding site number 1 of SPCAS9 with 17-Bp R-Loop Containing 2 Terminal Mismatches (State I - Pre-Activation) within 5.0Å range: probe atom residue distance (Å) B Occ A:Mg1401 b:85.0 occ:1.00 ND1 A:HIS983 2.7 83.9 1.0 OD2 A:ASP10 2.9 94.7 1.0 OD2 A:ASP986 3.4 91.9 1.0 CE1 A:HIS983 3.4 83.3 1.0 CG A:HIS983 3.8 74.2 1.0 CA A:HIS983 3.9 68.5 1.0 CD2 A:HIS982 3.9 73.4 1.0 CG A:ASP10 4.0 91.4 1.0 CB A:HIS983 4.2 69.7 1.0 NH2 A:ARG976 4.3 110.5 1.0 CG A:ASP986 4.3 86.8 1.0 NE2 A:HIS982 4.4 78.9 1.0 N A:HIS983 4.4 67.0 1.0 CB A:ASP986 4.5 73.2 1.0 O A:HIS982 4.5 74.9 1.0 OD1 A:ASP10 4.5 90.6 1.0 NE2 A:HIS983 4.6 77.6 1.0 C A:HIS982 4.7 67.4 1.0 CD2 A:HIS983 4.8 71.4 1.0 OG A:SER15 4.8 79.0 1.0 NZ A:LYS974 5.0 88.4 1.0 C A:HIS983 5.0 67.7 1.0

K.A.Kiernan, D.W.Taylor. Kinetics-Guided Cryo-Em Enables Visualization of CAS9 Dissociation Post-Cleavage To Be Published.